✔ Serves research needs ✔ Products with scientific potential ✔ Step-by-step support Protein A gold is a versatile colloidal gold conjugated immuno-reactive typically used for on-section immunogold labeling. Protein A…
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Double labeling kit Protein A 6nm / Protein A 15nm
The Double labeling kit Protein A 6nm / Protein A 15nm contains: 1 ml of Protein A 6nm Immuno Gold Reagent 1.4 ml of Protein A 15nm Immuno Gold Reagent…
The immuno incubation protocol
…an incubation with excess free Protein A or G (50-100 µg/ml). This will block practically all binding sites for Protein A or G. Next, incubate for the second antigen with…
Conventional Kit Protein A
The Conventional Kit Protein A contains: 1 ml of a 6 nm or 10 nm Conventional Protein A Immuno Gold Reagent or 1.4 ml of a 15 nm or 25…
Reactivity of Protein A and G
Membrane labeling with protein A 15 nm gold conjugate. Courtesy of T.Wakefield, University of Auburn, Alabama Species Ig(sub)class Protein A Protein G Rabbit Ig ++ ++ Mouse IgG1 – +…
Protein A
AURION Conventional Immunogold Reagents are tailored to contain 10-20 µg of specific protein/ml. The reagents are supplied in PBS with 1% Bovine Serum Albumin and 15 mM NaN3 at an…
Basic Blocking solution (for e.g. protein A and G)
AURION Blocking Solutions are prepared using specially selected compounds. All ruminant proteins are obtained from healthy livestock. AURION Blocking solutions contain Bovine Serum Albumin and Cold Water Fish Skin Gelatine…
Protocol Conventional Reagents
…For double marking using protein A or protein G gold conjugates each labeling is worked out separately. An incubation with free protein-A or protein-G at a concentration of 20-100 µg/ml…
BSA/CWFS/Tween 20/Normal Sera
…A and Protein G gold reagents. Application instructions The following recipes have been tried succesfully by researchers in the field and by Aurion: Blocking Solution for protein A or G…
Normal Rabbit Serum
…the same as the secondary antibody species (e.g. use normal goat serum with goat-anti-rabbit conjugates). Note: normal sera should not be used in combination with Protein A and Protein G…