To prepare a high quality (immuno)gold conjugate gold nano particles with uniform size and a highly adsorptive surface should be used. A narrow size distribution minimizes variation in your immuno assay and warrants low background levels.
Aurion Diagnostics Gold Nanoparticles are prepared according to unique production protocols based on the reduction with citrate. This provides for gold nanoparticles with the same quality as our gold nanoparticles for research applications.
Aurion gold nanoparticles for Diagnostics are available in two diameters: 20nm and 40nm. With these two particle sizes both adsorption and covalent conjugation methods are optional. For more information on gold nanoparticle conjugation strategies see our Newsletter #6. The coefficient of variance of Aurion Diagnostics Gold Nanoparticles is <12%. Aurion Diagnostics Gold Nanoparticles are available as a citrate stabilized gold sol for conjugation via adsorption as well as pre-functionalized with carboxyl end-groups for covalent conjugation via sulfo-NHS/EDC chemistry.
Aurion Diagnostics Gold Nanoparticles – Gold Sols – have been developed for conjugate preparation via the classic direct adsorption method.
More on Aurion Diagnostics Gold Nanoparticles – Gold Sols –
Gold Nanoparticles – Carboxyl Functionalized – have been developed to facilitate conjugating gold nanoparticles to molecules that cannot be conjugated via the classic direct adsorption method. The product is especially suited for covalent conjugation of small ligands.
More on Aurion Diagnostics Gold Nanoparticles – Carboxyl Functionalized –