AURION Newsletter 3
IN SITU HYBRIDIZATION for LIGHT and ELECTRON MICROSCOPY using ULTRA SMALL GOLD DETECTION and SILVER ENHANCEMENT
O. Sibon
Topics
– Introduction to in situ hybridization with gold conjugates
– Post- and pre-embedding approaches
– Structural preservation
– Opening up the nuclear matrix
– Comparison between alkaline phosphatase detection and IGSS detection at the light microscope level
– Schematic overview of critical steps in pre- and post-embedding procedures
– Detailed description of procedures
– Evaluation at the light microscope level
– How do post- and pre-embedding results compare?
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