AURION Newsletter 5
ULTRA SMALL GOLD REAGENTS and R-GENT SE-EM in PRE-EMBEDDING SINGLE and DOUBLE LABELING
Hong Yi and Jan L.M. Leunissen
This newsletter focuses on ImmunoGold Silver Staining in Pre-embedding labelling. Several examples illustrate the features of this approach both in single – and for the first time – also in double labeling using sequential silver enhancement,
Topics
– Evaluation of pre-embedding labeling
– Morphological preservation through the use of Aurion ECS
– Single labeling procedure
– Double labeling procedure
– Comprehensive and detailed description of methods
Pre-embedding double labeling of GFAP (small particles) and synaptophysin (large particles) in brain tissue using two ultrasmall gold conjugates and silver enhancement. Courtesy Mrs. Hong Yi, Emory University Atlanta
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