AURION Newsflyer 2 – part 1
DOUBLE LABEL IMMUNOHISTOCHEMISTRY for the SEPARATE OBSERVATION of TWO ANTIGENS using EPI-POLARIZATION MICROSCOPY for the IMMUNOGOLD-SILVER TECHNIQUE and FLUORESCENCE MICROSCOPY for the ALKALINE PHOSPHATASE STAINING
C.M. van der Loos
Topics
– Evaluation of double staining techniques
– Fluorescent and enzyme markers
– Discrimination between signals and implications for photography
– Fluorescent properties of alkaline phosphatase reaction products
– Suitability of Gold/Silver staining in combination with alkaline phosphatase staining
– Epi-polarization microscopy and fluorescent signal: two signals without overlap
– Detailed description of protocols
AURION Newsflyer 2 – part 2
A COMPARISON between the STREPTAVIDIN-BIOTIN-ALKALINE PHOSPHATASE METHOD and the IMMUNOGOLD TECHNIQUE using ULTRA SMALL GOLD PARTICLES and SILVER ENHANCEMENT
R. Willemsen
Topics
– Introduction to immunohistochemistry screening on blood smears
– Suitability of Gold/Silver staining and alkaline phosphatase staining
– Comparison of results
– using alkaline phosphatase staining (3 step approach)
– using immunogold silver staining (2 step and 3 step approaches)
– Detailed description of protocols on blood smears
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