General incubation protocol
1. Low Molecular Weight Block Step
2. High Molecular Weight Block Step
using AURION Blocking Solutions
3. Wash step
All wash steps use the recommended incubation solution, containing AURION BSA-c™
4. Primary antibody incubation
in recommended incubation solution, containing AURION BSA-c™
5. Wash steps
see first wash step
6. Immuno Gold conjugate incubation
using AURION Ultra Small Immuno Gold Reagents or AURION Conventional Reagents
7. Wash steps
see first wash step
8. Further wash steps
see first wash step
9. Silver Enhancement
for LM: AURION R-Gent SE-Intense
for EM: AURION R-Gent SE-EM
10. Wash steps
see first wash step
11. Contrasting or Staining
AURION – STEP BY STEP YOUR PARTNER IN RESEARCH