In many cases double labeling experiments are based on using two primary antibodies from different animal species, for instance mouse and rabbit. Of late we noticed that an increasing number of users have primary antibodies developed in Goat. It was impossible to perform a double label experiment in combination with for instance a mouse primary antibody, since applicable secondary antibodies are either raised in Goat or in Rabbit. Hence the Goat-anti-mouse would be recognized by the Rabbit-anti-Goat, and it would be impossible to get reliable results. In the past, only using directly labeled primary antibodies would solve this. And this would require researchers to go through the lengthy procedure of preparing such conjugates, and then often with very limited success.
To overcome this difficulty Aurion has developed a new series of secondary immunogold conjugates based on antibodies raised in Donkey, including Donkey-anti-Goat. It will be obvious that mixing a Donkey-anti-Goat and a Donkey-anti-Mouse will provide a successful approach in the aforementioned case.