KITS BASED ON THE AURION METHOD
A successful immuno-detection experiment stands or falls with the use of products of high quality that, when used in combination with each other, will lead to the best results.
Aurion supplies a comprehensive line of immuno gold and auxiliary reagents: conventional gold conjugates as well as signature products such as sub-nanometer Ultra Small immuno gold conjugates, R-Gent SE-EM, an electron microscopy grade silver enhancement reagent and Aurion BSA-c™, an incubation buffer additive for minimizing background. Together with a high affinity primary antibody this will give you the tools for a successful immuno-detection experiment.
Based on the extensively tested Aurion method, Aurion has developed incubation protocols for immuno gold (silver) staining for various applications, such as LM or EM, pre-embedding and post-embedding. All products applied in synergistic combination as described in the incubation protocols lead to clean, clear, reproducible and reliable results: The Aurion method. This method is also the basis for the Aurion Masterclasses and Workshops on Immuno Gold (Silver) Staining.
As our products are specifically designed to give optimum results when used in combination with each other Aurion offers you the option to order the individual Aurion reagents in a kit. The kit contains all you need to achieve the best immuno gold (silver) staining possible: blocking reagent, BSA-c (10%) buffer additive, the gold nanoparticle conjugated antibody and the silver enhancement reagents of your choice. The Aurion kits are available for all sizes of our immuno gold conjugates, and ordering the suggested combination of products as a kit also gives you a 10% discount compared to the prices of the individual components.
The Immuno Gold Silver Staining kit for Ultra Small and 2nm reagents contains:
– 1 small package vial of an Ultra Small (< 0.8nm) or 2 nm Immuno Gold Reagent
– 30 ml AURION BSA-c™ (10%)
– 30 ml AURION R-Gent SE-EM, silver enhancement reagents for Electron Microscopy or 60 ml AURION R-Gent SE-Intense, silver enhancement reagents for Light Microscopy
– 30 ml Blocking solution
The Immuno Gold Staining kit for Conventional reagents contains:
– 1 small package vial of a Conventional Immuno Gold Reagent
– 30 ml AURION BSA-c™ (10%)
– 30 ml Blocking solution
AURION Immunogold Double Labeling kits
The Aurion Method kits are now extended with a series of kits for double labeling experiments with different combinations of secondary antibody gold reagents often being used in double labeling experiments. For ease of discrimination between the two antigenic sites, the combination of 6nm and 15nm gold nanoparticles has been depicted.
AURION Ultra Small Starter kits
To make yourself familiar with our Ultra Small Gold Conjugates AURION has introduced the starter kit. This kit is sufficient for approximately 50 specimens.
An Ultra Small Starter Kit contains:
– 100 µl Ultra Small Immuno Gold reagent
(either Goat-anti-Rabbit IgG (H&L) Ultra Small or Goat-anti-Mouse IgG (H&L) Ultra Small)
– 5 ml AURION R-Gent SE-Intense (silver enhancement reagents for LM)
– 3 ml AURION R-Gent SE-EM (silver enhancement reagents for EM)
– 10 ml AURION BSA-c™ (10%)
If you require another Ultra Small Immuno gold reagent, you can use product code 708.000.
This Ultra Small Starter Kit contains:
– 100 µl Ultra Small ImmunoGold reagent of your choice
– 5 ml AURION R-Gent SE-Intense (silver enhancement reagents for LM)
– 3 ml AURION R-Gent SE-EM (silver enhancement reagents for EM)
– 10 ml AURION BSA-c™ (10%)
Because of the variable we cannot provide these kits via the webshop, therefore orders via e-mail, fax or telephone only. You can find more information on ordering on this web page.
Ordering information
When ordering, do not forget to specify which immuno gold reagent you require. For example, if you would like to order an Ultra Small Starter kit containing a Goat-anti-Rabbit IgG (H&L) Ultra Small gold conjugate, you indicate on your order: 708.000 (code of the kit) : 800.011 (Goat-anti-Rabbit IgG Ultra Small). You can choose from these Ultra Small Immuno Gold Reagents: https://aurion.nl/products/product-overview/quick-overview-ultra-small-reagents/?preview=true