The signal-to-noise ratio determines the quality of any detection experiment. This ratio is in principle determined by the characteristics of specimen and detection reagents. With adequate blocking and incubation media, background reactions are minimized whereas specific reactions are not hampered.
Aurion has developed Blocking Solutions which effectively block ‘sticky’ surfaces by multi-point hydrophobic and charge-based interactions. The Blocking Solutions are specifically designed to meet the characteristics of each type of secondary conjugate.
BSA/CWFS/Tween 20/Normal Sera
Many compounds have been added empirically to immunolabeling solutions for the purpose of minimizing background staining. Based on years of experience and controlled testing, Aurion has selected a group of compounds that are proven to be the most effective in their background reduction action. Researchers can obtain these items separately from Aurion and prepare blocking and incubation media to suit the needs of their own protocols.
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