Col-Aurion is a total protein stain consisting of a solution of coated gold particles with an average particle diameter of 15 nm. The staining principle is based on the electrostatic binding of negatively charged gold particles to proteins with a positive charge present at low pH.
A unique feature of Col-Aurion is the use of BSA-c™ to shield off the surface of the gold particles. Destabilization of gold particles that might occur as a result of interaction with detached protein is thus prevented. The strong negative charge of BSA-c™ gives an additional increase in sensitivity of the stain.
Col-Aurion total protein stain has a pH of 3.2 and is ready to use. For removal of surplus of weakly bound protein from the membrane, a 10 ml vial of Tween-20™ is included.