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Immunodetection methods

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Immunodetection methods are used to identify specific proteins in cells, tissue and in analytical techniques like immunoblotting. This technique requires the use of gold labeled immunodetection reagents, of which Aurion offers an extensive, ready-to-use range. We can, however, also provide you with an in-depth explanation of the required conjugation protocol and methods, enabling you to manufacture gold nanoparticle conjugated ligands and immunodetection reagents by yourself.

More information about methods of immunodetection

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Example: IGSS of alpha-amylase using GAR 2nm and AURION R-Gent SE-EM
Light microscopy evaluation of tubulin labeling with Ultra Small Immunogold Reagents and silver enhancement. Upper panel: bright field mode Lower panel: epi-polarization mode

Immunodetection protocol methods for immunogold and/or immunogold silver staining

Main steps in the methods of the immunodetection protocol are blocking, washing, primary antibody incubation, immunogold conjugate incubation and if appropriate, silver enhancement. Immunodetection is possible using post-embedding or pre-embedding methods, each requiring a specific incubation protocol. In general, a two-step immunodetection protocol is used. As ultra-small gold conjugates require a different approach to conventional-sized conjugates, the two immunodetection protocols are described in-depth in our ‘Incubation protocols’ section.

Expand your knowledge by participating in a Masterclass or in-house training

If you seek to expand your knowledge on both theoretical and practical aspects of immunodetection via immunogold labeling, Aurion offers several workshops every year for researchers around the world. Bring your own ready-to-use specimens and primary antibodies to learn, practice, and evaluate labeling under the guidance of an expert. You can also request a custom workshop to be organized in your own facility, adjusted to your personal requirements. Contact us today via info@aurion.nl or (+31) 317 415094 to register for our workshops or with any questions you have regarding the proper immunodetection protocols and methods.