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Silver enhancement reagents

Aurion specializes in advanced techniques and reagents for light and electron microscopy, including silver enhancement reagents for the immunogold silver staining method. With this method, metallic silver is deposited at the site of colloidal gold labelling with very little non-specific background deposition.

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Pre-embedding labeling on 2%PFA/GA fixed and freeze thaw permeabilized organotypic cultured brain slices using rabbit anti GFP, AURION GAR 2nm and R-Gent SE-EM. Courtesy of Y. Sun, D. Guerrero-Given and N. Kamasawa; EM Core Facility, Max Planck Florida, Institute for Neuroscience, Jupiter, FL USA
Light microscopy evaluation of tubulin labeling with Ultra Small Immunogold Reagents and silver enhancement. Upper panel: bright field mode Lower panel: epi-polarization mode

Silver enhancement reagents for immunogold silver staining

The main features of our specially developed R-Gent SE-EM Silver Enhancement reagent are efficiency and homogeneity. The reagent has reduced light sensitivity, so no special precautions are needed. Aurion R-Gent SE-EM is well-suited for pre-embedding immunogold silver staining due to its low viscosity. Moreover, these silver reagents have been extensively tested for use with Aurion’s ultra-small immunogold reagents to ensure optimal results. The R-Gent SE-LM Silver Enhancement kit will generate a brown to black signal that can be combined with most common counterstaining techniques like H&E or nuclear fast red. The use of reflected light microscopy will increase the sensitivity of detection of the immunogold silver staining method. Do you want to elaborate more on the technique? Aurion provides workshops to those who want to obtain a better understanding of the theoretical and practical aspects of the silver staining method. We also enlighten you about the part our reagents play in optimizing the immunogold silver staining technique and other immunohistochemistry protocols.

Be assured of a fast delivery when ordering reagents

Reagents for immunogold silver staining with enhanced efficiency, reduced light sensitivity, and ease of handling can be ordered directly from Aurion’s webshop. The silver enhancement reagents can be delivered from stock within 3 working days. Do not hesitate to contact us with your questions about immunodetection methods or to request a quotation. Call (+31) 317 415094 or send an email to info@aurion.nl.