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Immunohistochemistry protocol

Following the correct steps in the immunohistochemistry protocol is important to obtain optimal signal to noise ratios. Aurion has developed special reagents for this purpose, more specifically BSA-c and R-Gent SE-LM. This reagent develops the signal obtained with the immunogold staining protocol to make it visible in bright field light microscopy. We provide researchers with an in-depth explanation of the proper protocols in light microscopy for pre- and post-embedding immunogold labeling.

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Example: IGSS of alpha-amylase using GAR 2nm and AURION R-Gent SE-EM
Light microscopy evaluation of tubulin labeling with Ultra Small Immunogold Reagents and silver enhancement. Upper panel: bright field mode Lower panel: epi-polarization mode

Post-embedding and pre-embedding immunogold silver staining technique

In immunohistochemistry, the immunogold silver enhancement and staining technique is a good alternative for methods using enzyme markers. A permanent signal will be generated when using ultra-small gold conjugates, BSA-c buffer additive and R-Gent SE-LM. The signal can be combined with general counter-staining methods. To fully benefit from the advantages of the immunogold silver staining method, we offer high-quality, ready-to-use ultra-small gold conjugated antibodies suited for the immunohistochemistry labeling protocol. We offer an in-depth overview of the necessary incubation steps in our ‘Incubation protocols’ section. Here, you can find the correct protocols for both conventional immunogold reagents and ultra-small reagents for light microscopy. The section covers both the post-embedding and the pre-embedding immunohistochemistry protocol.

Learn more about immunogold staining during a workshop

If you would like to expand your knowledge of, and experience with, the immunogold staining technique, Aurion organizes several workshops around the world where researchers can bring their own primary antibodies and ready-to-use specimens to learn, practice, and evaluate the labeling protocol. As an alternative, you have the option for a custom workshop in your own facility which will be adapted to your personal requirements.

Contact us to participate in a workshop or for more information

Contact us today if you wish to participate in one of our workshops. Next to the immunohistochemistry protocol, the complete theory on immunogold conjugation and immunogold labeling methods for electron microscopy will be covered. Please contact us for any questions about our products and their applications. Call us on (+31) 317 415094 or send an email to info@aurion.nl.