Immunogold secondary antibodies of highest quality are available from Aurion. In our company, we are specialized in designing and manufacturing gold labeled antibodies for use in electron microscopy, light microscopy and in bio-assays. All of our reagents are for research purposes only. Our conventional immunogold reagents are available in four different particle diameters. They are well suited for multiple labeling without overlap thanks to their monodisperse size population.
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Immunogold secondary antibodies with wide range of antigen specificities
Conventional immunogold labeling relies in general on the use of secondary gold labeled antibodies. The gold nanoparticles used can be directly visualized in the electron microscope. In general, a silver enhancement is not needed. A good alternative to immunogold secondary antibodies is Protein A gold. Aurion’s conventional gold reagents are built around gold nanoparticles with a diameter of 6, 10, 15 or 25 nm. Conventional secondary antibody gold conjugates are available in a wide range of antigen specificities, such as:
The antibodies we use for conjugation are from goat, donkey, and rabbit, are affinity purified and cross-adsorbed.
Ultra-small immunogold reagents for a more efficient result
Conventional reagents are optimized for immuno electron microscopy applications. Aurion’s ultra-small immunogold reagents, prepared with subnanometer gold particles, give you superb results in all applications. Ultra-small conjugates behave similarly to uncoupled molecules. The small particle size will decrease steric hindrance. When used in conjunction with silver enhancement reagents, these secondary antibody gold conjugates provide optimal and efficient results independently of the visualization technique.
Participate in a workshop to gain more insight or order your reagents form the web shop
If you would like to gain a more detailed and in-depth understanding of the theoretical and practical aspects of immunogold labeling technique and the incubation protocols we recommend you to participate in one of our workshops. These unique workshops are hosted around the world for those researchers who would like to test our reagents in combination with their own ready-to-use specimens and specific primary antibodies. You may also order the immunogold secondary antibodies you require directly from our webshop. Most of them are in stock and can be delivered to you within 3 working days. Also, do not hesitate to call us with your questions at (+31) 317 415094 or send an email to firstname.lastname@example.org.