A gold nanoparticle conjugated antibody or gold conjugate can be used in a wide range of applications being immuno-electron microscopy, immunohistochemistry and bio-assays. The specific antigen of interest is visualized by the gold conjugate. This may be a one-step method having the gold conjugated direct to the primary antibody. In general, however, a two-step method is used where the specific primary antibody is detected by a second incubation with specific gold labeled (secondary) antibodies or e.g., protein A gold. Aurion specializes in designing and manufacturing high-quality gold reagents for use in immunogold and immunogold silver staining and offers gold conjugated antibodies with numerous antigen specificities for use in scientific research applications.
If you have any questions about our products you can go to our page dedicated to your questions.
Gold conjugate for use in immunoelectron microscopy and immunohistochemistry
In our product range, you can find gold labeled antibody conjugates with a variety of particle diameters. We have most specificities of gold conjugated antibodies in stock. Our conventional gold reagents are conjugated to gold nanoparticles in four particle diameters, ranging from 6 nm to 25 nm. The particle population is monodisperse. Minimal size variation and particle diameter overlap allows multiple labeling studies. These conventional-sized conjugates are the right choice for immunogold labeling when the antigen is abundant and the specimen offers good accessibility. However, the best choice for any application is that of our unique ultra-small gold reagents. As these gold nanoparticles have an average diameter of 0.8 nm or less, they have less influence on the adsorbed antibodies and behave as though they are uncoupled. This will have a positive effect on labeling density in general.
Custom conjugates for uncommon applications
Certain applications require a custom gold conjugate. Examples are gold labeled antibody or even fragments of the specific primary to improve resolution and gold nanoparticle conjugation of ligands for receptor-acceptor studies. To meet your research demands Aurion offers a custom labeling service. We operate in close cooperation with you to ascertain that the gold conjugate meets your specific requirements.
Unconjugated gold nanoparticles
Aurion is specialized in designing and manufacturing gold labeled antibodies and other immunoreactive gold conjugated (macro)molecules. We also offer you the possibility to gold conjugate yourselves. For this we have included colloidal gold sols in our product program. Either being citrate stabilized or carboxyl functionalized. The necessary protocols to create a gold conjugated antibody or gold conjugated ligand are explained in-depth on our website. If you wish to gain more knowledge and experience with regards to gold nano particle conjugation and immunogold silver staining, we also offer practical workshops .
Find the appropriate reagent for your application
Learn more about our reagents and which gold labeled antibody best suits your application in our ‘Sharing our knowledge’ and ‘Answering your question’ sections. Alternatively, you can immediately buy the appropriate gold labeled antibody from our web shop and have it be delivered to you within 3 working days. For more information or to request a custom gold conjugate, please call us +31 (0)317 415094 or send us an email.