Gold conjugate

Gold conjugate for use in immunoelectron microscopy and immunohistochemistry

In our product range, you can find gold labeled antibody conjugates with a variety of particle diameters. We have most specificities of gold conjugated antibodies in stock. Our conventional gold antibodies and reagents are conjugated to gold nanoparticles in four particle diameters, ranging from 6 nm to 25 nm. The particle population is monodisperse. Minimal size variation and particle diameter overlap allows multiple labeling studies. These conventional-sized conjugates are the right choice for immunogold labeling when the antigen is abundant, and the specimen offers good accessibility. However, the best choice for any application is that of our unique ultra-small gold reagents. As these gold nanoparticles have an average diameter of 0.8 nm or less, they have less influence on the adsorbed antibodies and behave as though they are uncoupled. This will have a positive effect on labeling density in general.

Custom gold conjugates

The standard series of Aurion gold nanoparticle conjugated immunoreagents is intended for the two and three step detection of antigens. Although these approaches cover the majority of applications, there may occasionally exist a need for a direct label where the gold nanoparticles are coupled directly to the primary antibody or, more commonly, to a protein or peptide with specific binding properties. An example is found in working with specimens where substantial amounts of homologous immunoglobulins are present. A further example is found in the detection of receptors through labeled ligand molecules.