Colloidal gold conjugated with secondary antibodies are available in gold nanoparticle diameters ultra-small and conventional sizes 6 to 25nm. The monodisperse size population makes conventional gold conjugated antibodies perfectly suited for multiple labeling studies. As an alternative colloidal gold can be purchased unconjugated or functionalized with a carboxyl group.
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Colloidal gold with secondary antibodies in different gold nanoparticle sizes
Our standard stock holds conventional colloidal gold with secondary antibodies in four particle diameters, ranging from 6 nm to 25 nm. These conventional-sized reagents are prepared using the highest quality of antibodies or binding agents, to provide you with the most trustworthy results in your research. However, we also offer unique ultra-small colloidal gold with secondary antibodies, which offer even more efficiency and better results in any immuno-application. Ultra-small colloidal gold conjugated antibodies resemble the unconjugated antibody. The conjugate carries less net negative charge in comparison to conventional gold conjugates and therefore less charge determined repulsion when approaching the sample surface. This has a beneficial effect on labeling density. In combination with silver enhancement reagents ultra-small gold conjugates can be used in electron microscopy, light microscopy and immunoblotting. Both conventional and ultra-small colloidal gold conjugated antibodies are available in a wide range of specificities, the full list of which can be found in our product overview.
Request a custom-label or order from stock
Do you want gold particles conjugated directly to the primary antibody or do you want a different (macro)molecule conjugated to gold nanoparticles? Aurion offers a custom labeling service that provides you with gold conjugate you need for your research application. Contact us at +31 (0)317 415094 or via email to request a custom labeling or order your colloidal gold conjugated antibody directly from our web shop.