Description
The AURION R-GENT SE-INTENSE components constitute a Silver Enhancement Reagent which increases the average gold cluster or particle size by deposition of metallic silver. The generated intense brown-black signal is compatible with counterstaining in light microscopy. The signal is also easily detected in bio assays. AURION R-GENT SE-INTENSE has been tailored for the enhancement of AURION Ultra Small and AURION 2nm Immunogold reagents and is equally suited for the larger sized particles in the AURION Conventional Immunogold reagents.
For enhancement, equal amounts of the DEVELOPER and ENHANCER are mixed well and applied to the specimen. The enhancement mixture is easy-to-use, exhibits extremely delayed auto-nucleation and can be used under standard laboratory light conditions. Typical enhancement times are between 15 and 25 minutes. Auto-nucleation becomes visible only after 35-45 minutes. Light microscopical specimens may be counterstained according to standard procedures. The enhancement mixture has a pH value of 8.3-8.5.
AURION R-GENT SE-INTENSE is available as a kit in two unit sizes (2 x 30 ml or 2 x 250 ml) and consists of a separate DEVELOPER and ENHANCER. The supplied amounts accomodate 600 and 5000 LM specimens respectively at 100 µl/specimen, or 60 and 500 bio assay specimens at 1 ml/specimen. The reactivity is tested on dot-spots and the absence of autonucleation is monitored by spectrophotometric techniques.
