AURION Enhancement Conditioning Solcution – ECS – is a concentrated buffer solution, improving immuno gold silver staining results. Aurion ECS is supplied as a 10x concentrate and is compatible with all the Aurion reagents.
Detailed information is provided in the package insert. See also Aurion Newsletter 5 as well as the info on double labelling on this website.
Used as a 10x dilution Aurion ECS serves as a washing medium after immuno incubation and before silver enhancement (immuno procedure – wash – silver enhancement).
In addition to this application in singe labelling it is required in double labelling experiments as a washing medium between the first silver enhancement step and the second immuno incubation sequence.
(immuno incubation1 – wash – silver enhancement1 – wash – immuno incubation 2 – wash – silver enhancement2).
Aurion ECS has a guaranteed shelf life of 18 months from the date of quality control analysis. Store at 4-8°C. Do not freeze.