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Gold nanoparticle conjugation

Different methods exist for gold nanoparticle conjugation of antibodies, as well other macromolecules, and low molecular weight ligands. This may be either conjugation via adsorption or gold nanoparticle conjugation via covalent binding. Basic principles, various advantages and disadvantages are explained in short on this page. Detailed information is presented in newsletter 6 which is published in the ‘Sharing our knowledge’ section of the website.

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Example: IGSS of alpha-amylase using GAR 2nm and AURION R-Gent SE-EM
Light microscopy evaluation of tubulin labeling with Ultra Small Immunogold Reagents and silver enhancement. Upper panel: bright field mode Lower panel: epi-polarization mode

Different options for antibody gold nanoparticle conjugation

Newsletter 6 in short

Which technique for conjugation to gold nanoparticles is preferred, depends on the characteristics of the molecules to be conjugates. In general, the adsorption technique appears to be the best choice for the conjugation of antibodies. However, as shown in our newsletter, a covalent binding is the method of choice to create a stable complex with low molecular weight molecules, for example the conjugation of Apolipoprotein-E to 6nm gold particles.

Request a custom gold nanoparticle conjugation or order our gold nanoparticles from stock

When your research requires a special gold conjugate, contact Aurion for custom covalent gold nanoparticles. Depending on the characteristics of your (macro)molecule and demand, our custom labeling service may use conventional gold sols, as well as our ultra-small gold nanoparticles. Each custom conjugation is performed in close cooperation with you, our customer. You can also order ready-to-use gold conjugated secondary antibodies, gold conjugated antibodies with different size diameters and antigen specificities from our extensive antibody stock. Our experts are always on hand to answer any questions regarding proper immunohistochemistry protocols and reagents. You can contact us at (+31) 317 415094 or info@aurion.nl.