Immunogold labeling

Conventional immunogold labeling

Our conventional immunogold reagents for immunogold staining are designed for use in immuno electron microscopy. The gold markers of these immunogold labeling agents can be seen directly with standard electron microscopic methods, meaning no additional silver enhancement step is required. These reagents are based on colloidal gold particles of assorted sizes, spanning from 6 nm to 25 nm, ensuring a monodisperse particle population. This minimizes size variation and particle diameter overlap, facilitating multiple labeling studies. Aurion immunogold reagents are formulated to meet various labeling protocol.

Ultra-small gold reagents for immunolabeling

In the domain of immunogold staining, our conventional immunogold conjugates are particularly effective for post-embedding immunogold labeling and detecting cell surface antigens that are readily accessible. Aurion also provides unique ultra-small gold reagents for immunogold staining. These particles, averaging 0.8 nm in diameter, can be directly visualized in high-angle annular dark-field scanning TEM. Typically, however, silver enhancement is necessitated for immunogold staining. This process deposits metallic silver on the gold nanoparticle surface, amplifying the diameter of ultra-small gold to levels visible in regular TEM, SEM, light microscopy, and bioassays. For electron microscopy observations, Aurion has developed R-GENT SE-EM, another specialized product.